Hotspot

ndhF-rpl32

SSC · 116484-117572 · 1089 bp

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Pi 0.00709
MarkerSeek score 59.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1089
variable_sites
21
pi
0.00709
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 40.0 45.0 57.0 57.8 1259-1285 bp 1.0 73.7
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 40.0 45.0 57.0 57.8 1257-1283 bp 1.0 73.3
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 40.0 55.0 57.0 58.3 1246-1272 bp 1.0 72.4
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 40.0 45.0 57.0 57.6 1256-1282 bp 1.0 71.5
ndhF-rpl32_p5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 38.1 45.0 57.7 57.8 1260-1286 bp 1.0 71.5