Hotspot

trnT-GGU-psbD

LSC · 34001-35490 · 1490 bp

Back to Amelanchier

Pi 0.00647

MarkerSeek score 52.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1490
variable_sites: 22
pi: 0.00647
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1519-1850 bp	1.0	84.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1522-1853 bp	1.0	84.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1521-1852 bp	1.0	84.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1509-1840 bp	1.0	84.3
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1524-1855 bp	1.0	84.1