Hotspot

trnR-UCU-atpA

LSC · 10111-10770 · 660 bp

Back to Amelanchier

Pi 0.0233
MarkerSeek score 58.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
660
variable_sites
35
pi
0.0233
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.90
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG ACAATCTCAATCATCCCCGC 50.0 50.0 58.0 58.0 1199-1500 bp 1.0 86.9
trnR-UCU-atpA_p2 CCTAGCCTTCCAAGCTAACG ACAATCTCAATCATCCCCGC 55.0 50.0 58.1 58.0 1202-1503 bp 1.0 86.6
trnR-UCU-atpA_p3 AGCCTTCCAAGCTAACGATG TTCGGTTTCCAGGGTAGGAT 50.0 50.0 58.0 58.0 1379-1680 bp 1.0 86.4
trnR-UCU-atpA_p4 AGCCTTCCAAGCTAACGATG TCAGACCGGCCATTAATGTG 50.0 50.0 58.0 58.0 1404-1705 bp 1.0 86.3
trnR-UCU-atpA_p5 CCTAGCCTTCCAAGCTAACG TCAGACCGGCCATTAATGTG 55.0 50.0 58.1 58.0 1407-1708 bp 1.0 86.1