Hotspot

petL

LSC · 66020-66115 · 96 bp

Back to Ambrosia

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label petL

Feature Metadata

label_name: petL
feature_type: gene
region: LSC
length_bp: 96
variable_sites: 3
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL_p1	AGGAAACTCAGGTAAGTGCT	ACACAGAAGTTCGCGAAAAG	45.0	45.0	56.1	57.0	214 bp	1.0	57.6
petL_p2	AAGGAAACTCAGGTAAGTGCT	ACACAGAAGTTCGCGAAAAG	42.9	45.0	56.9	57.0	215 bp	1.0	55.8
petL_p3	AGGAAACTCAGGTAAGTGCTT	ACACAGAAGTTCGCGAAAAG	42.9	45.0	56.9	57.0	214 bp	1.0	55.8
petL_p4	AGGAAACTCAGGTAAGTGCT	CACAGAAGTTCGCGAAAAGA	45.0	45.0	56.1	56.7	213 bp	1.0	55.3
petL_p5	AGGAAACTCAGGTAAGTGCT	ACACAGAAGTTCGCGAAAAGA	45.0	42.9	56.1	58.5	214 bp	1.0	54.1