Hotspot

ndhI-ndhG

SSC · 118477-118858 · 382 bp

Back to Ambrosia

Pi 0.0166

MarkerSeek score 53.60

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 382
variable_sites: 6
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TTGATCACATCCGAGCGTTT	AGACCACGGAAGTTACTCCA	45.0	50.0	57.9	58.0	1035-1055 bp	1.0	81.5
ndhI-ndhG_p2	TTGATCACATCCGAGCGTTT	AGACTTGTACAGACCACGGA	45.0	50.0	57.9	58.0	1045-1065 bp	1.0	81.5
ndhI-ndhG_p3	ATGGCCAACAAACAGTACGA	AGACCACGGAAGTTACTCCA	45.0	50.0	57.7	58.0	1142-1162 bp	1.0	79.9
ndhI-ndhG_p4	ATGGCCAACAAACAGTACGA	AGACTTGTACAGACCACGGA	45.0	50.0	57.7	58.0	1152-1172 bp	1.0	79.9
ndhI-ndhG_p5	TCAAATTGCTTTGGGTCGGT	AGACCACGGAAGTTACTCCA	45.0	50.0	58.3	58.0	780 bp	0.5	57.5