Hotspot

rpl14-rpl16

LSC · 81167-81294 · 128 bp

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Pi 0.0234

MarkerSeek score 66.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 3
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	243 bp	1.0	76.2
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	273 bp	1.0	76.0
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	236 bp	1.0	75.5
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	266 bp	1.0	75.3
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.5	57.4	225 bp	1.0	72.3