Hotspot

psbH-petB

LSC · 74789-74930 · 142 bp

Back to Ambrosia

Pi 0.0152

MarkerSeek score 65.30

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 2
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TATCCGACGAAGGAGAACCA	50.0	50.0	57.9	57.9	312-322 bp	1.0	81.1
psbH-petB_p2	CGTCGCAATGGCTCTATTTG	TATCCGACGAAGGAGAACCA	50.0	50.0	57.6	57.9	313-323 bp	1.0	79.0
psbH-petB_p3	GTCGCAATGGCTCTATTTGC	ATCCGACGAAGGAGAACCAT	50.0	50.0	57.9	58.5	311-321 bp	1.0	78.0
psbH-petB_p4	CGTCGCAATGGCTCTATTTG	ATCCGACGAAGGAGAACCAT	50.0	50.0	57.6	58.5	312-322 bp	1.0	75.9
psbH-petB_p5	GTCGCAATGGCTCTATTTGC	TCCGACGAAGGAGAACCAT	50.0	52.6	57.9	58.0	310-320 bp	1.0	73.9