Hotspot

psbN-psbH

LSC · 74465-74548 · 84 bp

Back to Ambrosia

Pi 0.0238

MarkerSeek score 66.20

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 84
variable_sites: 2
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	TGTTGAGAAGGTTGCCCAAA	CAGTAGTTCGTCTTGGACCAG	45.0	52.4	57.8	58.1	255 bp	1.0	71.0
psbN-psbH_p2	TTGTTGAGAAGGTTGCCCAA	CAGTAGTTCGTCTTGGACCAG	45.0	52.4	57.8	58.1	256 bp	1.0	71.0
psbN-psbH_p3	TGTTGAGAAGGTTGCCCAAA	CAGTAGTTCGTCTTGGACCA	45.0	50.0	57.8	56.9	255 bp	1.0	70.6
psbN-psbH_p4	TTGTTGAGAAGGTTGCCCAA	CAGTAGTTCGTCTTGGACCA	45.0	50.0	57.8	56.9	256 bp	1.0	70.6
psbN-psbH_p5	TGTTGAGAAGGTTGCCCAAA	ACAGTAGTTCGTCTTGGACC	45.0	50.0	57.8	56.9	256 bp	1.0	70.6