Hotspot

rbcL-accD

LSC · 56560-57139 · 580 bp

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Pi 0.0143

MarkerSeek score 64.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 8
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGGCTACCAAATGGAGTCCT	GACCAAGACTGCTCGTTGAT	50.0	50.0	58.0	57.9	787-792 bp	1.0	81.9
rbcL-accD_p2	GGCTACCAAATGGAGTCCTG	GACCAAGACTGCTCGTTGAT	55.0	50.0	58.0	57.9	786-791 bp	1.0	81.8
rbcL-accD_p3	AGGCTACCAAATGGAGTCCT	ATAGGACCAAGACTGCTCGT	50.0	50.0	58.0	58.1	791-796 bp	1.0	81.4
rbcL-accD_p4	GGCTACCAAATGGAGTCCTG	ATAGGACCAAGACTGCTCGT	55.0	50.0	58.0	58.1	790-795 bp	1.0	81.3
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GACCAAGACTGCTCGTTGAT	55.0	50.0	57.7	57.9	774-779 bp	1.0	79.9