Hotspot

trnE-UUC-rpoB

LSC · 12402-13327 · 926 bp

Back to Ambrosia

Pi 0.0376

MarkerSeek score 73.60

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 29
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.0	57.8	1010-1064 bp	1.0	84.4
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	CTTCTGCCAAACCCTGATCA	55.0	50.0	58.1	57.8	969-1023 bp	1.0	83.8
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.1	57.8	1000-1054 bp	1.0	83.6
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	CTTCTGCCAAACCCTGATCA	50.0	50.0	58.1	57.8	1005-1059 bp	1.0	83.6
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCTTCTGCCAAACCCTGATC	50.0	50.0	58.0	57.5	1011-1065 bp	1.0	82.1