Hotspot

trnY-GUA-trnE-UUC

LSC · 12151-12329 · 179 bp

Back to Ambrosia

Pi 0.0391
MarkerSeek score 66.40
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
179
variable_sites
7
pi
0.0391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC AAAACGAGGGTTCAAGTCCC 55.0 50.0 58.4 58.0 252 bp 1.0 79.2
trnY-GUA-trnE-UUC_p2 ACGAATTTACAGTCCGTCCC AAAACGAGGGTTCAAGTCCC 50.0 50.0 57.6 58.0 253 bp 1.0 79.0
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC CGGAGAAAACGAGGGTTCAA 55.0 50.0 58.4 58.1 257 bp 1.0 78.4
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC TTTCACGGAGAAAACGAGGG 55.0 50.0 58.4 58.1 262 bp 1.0 78.3
trnY-GUA-trnE-UUC_p5 CGAATTTACAGTCCGTCCCC CCCCTATGGTCCAGTGGTTA 55.0 55.0 58.4 58.1 293 bp 1.0 78.2