Hotspot

matK-trnK-UUU

LSC · 3757-4502 · 746 bp

Back to Ambrosia

Pi 0.0107

MarkerSeek score 64.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 8
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGATAAGCGGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	56.0	58.2	843 bp	1.0	63.1
matK-trnK-UUU_p2	ACTCCTGAAAGATAAGCGGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	56.0	58.2	842 bp	1.0	63.1
matK-trnK-UUU_p3	ACTCCTGAAAGATAAGCGGA	TTGGGTTGCTAACTCAACGG	45.0	50.0	56.0	58.4	858 bp	1.0	61.2
matK-trnK-UUU_p4	ACTCCTGAAAGATAAGCGGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	56.0	57.5	856 bp	1.0	60.7
matK-trnK-UUU_p5	ACTCCTGAAAGATAAGCGGAT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	56.5	58.2	842 bp	1.0	59.5