Hotspot

rps16

LSC · 5023-6135 · 1113 bp

Back to Amaranthus

Pi 0.00394

MarkerSeek score 56.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1113
variable_sites: 25
pi: 0.00394
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTGTGTGTTGGATTGGCACT	TGACTGTCCTGACTGAACCA	45.0	50.0	58.2	58.2	1310-1324 bp	1.0	82.2
rps16_p2	TTTGTGTGTTGGATTGGCAC	TGACTGTCCTGACTGAACCA	45.0	50.0	57.4	58.2	1311-1325 bp	1.0	79.3
rps16_p3	TGTGTGTTGGATTGGCACTA	TGACTGTCCTGACTGAACCA	45.0	50.0	57.4	58.2	1309-1323 bp	1.0	78.8
rps16_p4	TGTGTTGGATTGGCACTACA	TGACTGTCCTGACTGAACCA	45.0	50.0	57.4	58.2	1307-1321 bp	1.0	78.8
rps16_p5	TTGTGTGTTGGATTGGCACT	ACCGGTTCCAAATTTGAGGA	45.0	45.0	58.2	57.3	1253-1267 bp	1.0	78.6