Hotspot

psaJ-rpl33

LSC · 66726-67067 · 342 bp

Back to Amaranthus

Pi 0.0129

MarkerSeek score 61.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 20
pi: 0.0129
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	ATATCTGGAAACGCCCCTTG	45.0	50.0	58.0	57.7	474-478 bp	1.0	83.1
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	ATATCTGGAAACGCCCCTTG	45.0	50.0	58.1	57.7	465-469 bp	1.0	82.8
psaJ-rpl33_p3	TTGGGTCTTTAGCCGGTCTA	ATATCTGGAAACGCCCCTTG	50.0	50.0	58.1	57.7	513-517 bp	1.0	82.3
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	ATATCTGGAAACGCCCCTTG	50.0	50.0	58.0	57.7	553-557 bp	1.0	82.1
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCCG	ATATCTGGAAACGCCCCTTG	50.0	50.0	58.2	57.7	518-522 bp	1.0	81.4