Hotspot

petA-psbJ

LSC · 62750-63676 · 927 bp

Back to Amaranthus

Pi 0.00761

MarkerSeek score 56.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 32
pi: 0.00761
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCGCGTACAAGGTCTTTT	CTGGATTAGGCTCATCGCTG	47.4	55.0	58.0	58.2	1057-1070 bp	1.0	73.3
petA-psbJ_p2	CGCGTACAAGGTCTTTTGTT	CTGGATTAGGCTCATCGCTG	45.0	55.0	57.0	58.2	1055-1068 bp	1.0	73.1
petA-psbJ_p3	TGCGCGTACAAGGTCTTTT	TCTGGATTAGGCTCATCGCT	47.4	50.0	58.0	58.3	1058-1071 bp	1.0	72.6
petA-psbJ_p4	CGCGTACAAGGTCTTTTGTTC	CTGGATTAGGCTCATCGCTG	47.6	55.0	58.1	58.2	1055-1068 bp	1.0	72.6
petA-psbJ_p5	CGCGTACAAGGTCTTTTGTT	TCTGGATTAGGCTCATCGCT	45.0	50.0	57.0	58.3	1056-1069 bp	1.0	72.4