Hotspot

psbM-trnD-GUC

LSC · 29233-30306 · 1074 bp

Back to Amaranthus

Pi 0.0121

MarkerSeek score 59.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 70
pi: 0.0121
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTTTGGCTGACCGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.4	58.6	872-1272 bp	1.0	71.8
psbM-trnD-GUC_p2	AATCATTTTGGCTGACCGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	874-1274 bp	1.0	69.9
psbM-trnD-GUC_p3	ATCATTTTGGCTGACCGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.2	58.6	873-1273 bp	1.0	69.9
psbM-trnD-GUC_p4	TCATTTTGGCTGACCGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	57.2	58.6	872-1272 bp	1.0	69.8
psbM-trnD-GUC_p5	TTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.1	58.6	868-1268 bp	1.0	69.0