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trnC-GCA-petN

LSC · 27642-28143 · 502 bp

Back to Amaranthus

Pi 0.014

MarkerSeek score 59.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 26
pi: 0.014
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTTCCCCAGTTCAAATCCG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.1	58.3	548-577 bp	1.0	82.4
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	GCCCAAGCGAGACTTACAAT	45.0	50.0	57.9	58.3	603-632 bp	1.0	82.3
trnC-GCA-petN_p3	TGTTCCCCAGTTCAAATCCG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	610-639 bp	1.0	81.2
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	665-694 bp	1.0	81.1
trnC-GCA-petN_p5	GCAAATCCTTGTTCCCCAGT	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.4	58.3	557-586 bp	1.0	80.2