Hotspot

trnS-trnG-UCC

LSC · 8062-8718 · 657 bp

Back to Amaranthus

Pi 0.02

MarkerSeek score 65.60

Primer available yes

Label trnS-trnG-UCC

Feature Metadata

label_name: trnS-trnG-UCC
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 47
pi: 0.02
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TCAAACCGGTAGACCCCTTA	50.0	50.0	58.2	57.7	788-851 bp	1.0	85.0
trnS-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACCGGTAGACCCCTTAACT	50.0	50.0	58.2	57.7	785-848 bp	1.0	84.9
trnS-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AATCAAACCGGTAGACCCCT	50.0	50.0	58.2	58.3	790-853 bp	1.0	84.9
trnS-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATCAAACCGGTAGACCCCTT	50.0	50.0	58.2	58.3	789-852 bp	1.0	84.9
trnS-trnG-UCC_p5	TGGAAACGGAAAGAGAGGGA	TCAAACCGGTAGACCCCTTA	50.0	50.0	58.3	57.7	793-856 bp	1.0	83.9