Hotspot

trnK-UUU-rps16

LSC · 4174-5022 · 849 bp

Back to Amaranthus

Pi 0.0183

MarkerSeek score 60.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 59
pi: 0.0183
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.3	58.1	859-966 bp	1.0	86.3
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	CAAGAGGGCGGAGGTTTTTA	50.0	50.0	58.3	57.8	864-971 bp	1.0	85.4
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	AGAGGGCGGAGGTTTTTAAG	50.0	50.0	58.3	57.5	862-969 bp	1.0	83.1
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GAGGGCGGAGGTTTTTAAGG	50.0	55.0	58.3	58.5	861-968 bp	1.0	82.7
trnK-UUU-rps16_p5	CGAATTCCTTGCTTCATCCG	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	57.3	58.1	898-1005 bp	1.0	82.4