Hotspot

rps16

LSC · 4719-5838 · 1120 bp

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Pi 0.00371
MarkerSeek score 62.50
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1120
variable_sites
15
pi
0.00371
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGATGCTAGGACCCTCTCTT ACATATCAGTTGATCCAATGACT 50.0 34.8 56.8 55.4 1208-1292 bp 1.0 45.2
rps16_p2 AGATGCTAGGACCCTCTCTT TCAGTTGATCCAATGACTATTCA 50.0 34.8 56.8 55.4 1203-1287 bp 1.0 45.2
rps16_p3 AGATGCTAGGACCCTCTCTT TGAATCAATTCCATATCGGTTTTGA 50.0 32.0 56.8 57.4 1169-1253 bp 1.0 45.2
rps16_p4 AGATGCTAGGACCCTCTCTT TCAATTCCATATCGGTTTTGA 50.0 33.3 56.8 53.2 1165-1249 bp 1.0 45.2
rps16_p5 AGATGCTAGGACCCTCTCTT CAGTTGATCCAATGACTATTCATGA 50.0 36.0 56.8 57.2 1202-1286 bp 1.0 45.2