Hotspot

rps15-ycf1

SSC · 120648-121075 · 428 bp

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Pi 0.0157
MarkerSeek score 53.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
428
variable_sites
16
pi
0.0157
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCGTCTTGCGGAATAACTGA ACCCATAACAGCAGTCGTTTT 45.0 42.9 57.0 58.1 511-535 bp 1.0 69.6
rps15-ycf1_p2 TCGTCTTGCGGAATAACTGA ACCCATAACAGCAGTCGTTT 45.0 45.0 57.0 57.4 511-535 bp 0.9 69.3
rps15-ycf1_p3 TCGTCTTGCGGAATAACTGA GGCCCAATTACCGATTAGAGG 45.0 52.4 57.0 58.2 564-588 bp 1.0 68.7
rps15-ycf1_p4 TCGTCTTGCGGAATAACTGAA ACCCATAACAGCAGTCGTTT 42.9 45.0 57.7 57.4 511-535 bp 0.9 67.0
rps15-ycf1_p5 TTCGTCTTGCGGAATAACTGA ACCCATAACAGCAGTCGTTT 42.9 45.0 57.7 57.4 512-536 bp 0.9 67.0