Hotspot

ndhF-rpl32

SSC · 109864-110547 · 684 bp

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Pi 0.0106
MarkerSeek score 66.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
684
variable_sites
20
pi
0.0106
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATAAAGGGT ACTGCCGTTAAATACCCCTT 45.0 45.0 54.6 56.8 727-827 bp 1.0 49.5
ndhF-rpl32_p2 CTGGAAGTGGAATAAAGGGT ACTGCCGTTAAATACCCCTTT 45.0 42.9 54.6 57.6 727-827 bp 1.0 47.4
ndhF-rpl32_p3 AGGGTATTATCCATGCGTATTGA ACTGCCGTTAAATACCCCTT 39.1 45.0 57.2 56.8 712-812 bp 1.0 46.6
ndhF-rpl32_p4 AAAGGGTATTATCCATGCGT ACTGCCGTTAAATACCCCTT 40.0 45.0 54.5 56.8 714-813 bp 0.9 43.8
ndhF-rpl32_p5 AAAGGGTATTATCCATGCGT ACTGCCGTTAAATACCCCTTT 40.0 42.9 54.5 57.6 714-813 bp 0.9 41.8