Hotspot

psbE-petL

LSC · 62822-63874 · 1053 bp

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Pi 0.0103
MarkerSeek score 59.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1053
variable_sites
34
pi
0.0103
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.9 58.0 1210-1232 bp 1.0 74.5
psbE-petL_p2 CAACCCGCAATGAATAGGGA TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1208-1230 bp 1.0 74.5
psbE-petL_p3 CAACCCGCAATGAATAGGGA ACCAATCAATAGAGCTGGGG 50.0 50.0 57.9 57.0 1223-1245 bp 1.0 74.1
psbE-petL_p4 CAACCCGCAATGAATAGGGA CCAATCAATAGAGCTGGGGT 50.0 50.0 57.9 57.0 1222-1244 bp 1.0 74.1
psbE-petL_p5 ACTGACGAATAACCAACCCG TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1221-1243 bp 1.0 74.1