Hotspot

petA-psbJ

LSC · 61391-62042 · 652 bp

Back to Amana

Pi 0.0179
MarkerSeek score 64.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
652
variable_sites
29
pi
0.0179
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAATTGTACGA GGTTCATATTCCGGGTTGGG 33.3 55.0 53.7 58.3 701-726 bp 1.0 46.7
petA-psbJ_p2 TGAAAAGGTTCAATTGTACGA CATATTCCGGGTTGGGTTCA 33.3 50.0 53.7 57.6 697-722 bp 1.0 46.7
petA-psbJ_p3 TGAAAAGGTTCAATTGTACGA GTTCATATTCCGGGTTGGGT 33.3 50.0 53.7 57.6 700-725 bp 1.0 46.7
petA-psbJ_p4 TGAAAAGGTTCAATTGTACGAA GGTTCATATTCCGGGTTGGG 31.8 55.0 54.5 58.3 701-726 bp 1.0 46.7
petA-psbJ_p5 AGAAACAGTTTGAAAAGGTTCA GGTTCATATTCCGGGTTGGG 31.8 55.0 54.6 58.3 711-736 bp 1.0 46.6