Hotspot

ndhC-trnV-UAC

LSC · 48586-49559 · 974 bp

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Pi 0.0057
MarkerSeek score 61.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
974
variable_sites
20
pi
0.0057
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCCGGCCCTTCACTAATTG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1112-1134 bp 1.0 82.6
ndhC-trnV-UAC_p2 GCCCTTCACTAATTGGTGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1107-1129 bp 1.0 82.4
ndhC-trnV-UAC_p3 CTTTTCCGGCCCTTCACTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1115-1137 bp 1.0 82.1
ndhC-trnV-UAC_p4 TTCCGGCCCTTCACTAATTG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1111-1133 bp 1.0 79.9
ndhC-trnV-UAC_p5 GCCCTTCACTAATTGGTGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.2 57.5 1106-1128 bp 1.0 79.6