Hotspot

ycf3-trnS-GGA

LSC · 42589-43166 · 578 bp

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Pi 0.00696
MarkerSeek score 64.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
578
variable_sites
15
pi
0.00696
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTCCCCTGAAGTTGTCGG CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.0 58.1 729-744 bp 1.0 82.8
ycf3-trnS-GGA_p2 TTTTCCCCTGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 750-765 bp 1.0 82.3
ycf3-trnS-GGA_p3 TTTTCCCCTGAAGTTGTCGG TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 700-715 bp 1.0 82.2
ycf3-trnS-GGA_p4 CCCCTGAAGTTGTCGGAATT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 725-740 bp 1.0 81.4
ycf3-trnS-GGA_p5 CCCCTGAAGTTGTCGGAATT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 746-761 bp 1.0 80.9