Hotspot

psbK-psbI

LSC · 7299-7487 · 189 bp

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Pi 0.0401
MarkerSeek score 69.00
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
189
variable_sites
15
pi
0.0401
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.88
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GCTGCTGTAAGTTTTCGATGA GGATTGCGTCCTGGATCATT 42.9 50.0 56.9 58.0 281-305 bp 1.0 71.5
psbK-psbI_p2 GCTGCTGTAAGTTTTCGATGA TTCTTCACGTCCAGGATTGC 42.9 50.0 56.9 58.2 294-318 bp 1.0 70.0
psbK-psbI_p3 GCTGCTGTAAGTTTTCGATG GGATTGCGTCCTGGATCATT 45.0 50.0 55.4 58.0 281-305 bp 1.0 67.4
psbK-psbI_p4 GCTGCTGTAAGTTTTCGATG TTCTTCACGTCCAGGATTGC 45.0 50.0 55.4 58.2 294-318 bp 1.0 65.9
psbK-psbI_p5 GCTGCTGTAAGTTTTCGATGA GGATTGCGTCCTGGATCATTA 42.9 47.6 56.9 57.9 281-305 bp 1.0 62.8