Hotspot

trnQ-UUG-psbK

LSC · 6784-7106 · 323 bp

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Pi 0.00764
MarkerSeek score 61.30
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
323
variable_sites
10
pi
0.00764
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GAAGGATTCGAACCTCCGAG AAAAAGCATAAGCCTCGGG 55.0 47.4 57.8 55.9 483-503 bp 1.0 56.5
trnQ-UUG-psbK_p2 TTGATTCTGAGGGTCTGGGA AAAAAGCATAAGCCTCGGG 50.0 47.4 57.7 55.9 505-525 bp 1.0 55.4
trnQ-UUG-psbK_p3 TGATTCTGAGGGTCTGGGAC AAAAAGCATAAGCCTCGGG 55.0 47.4 58.4 55.9 504-524 bp 1.0 54.8
trnQ-UUG-psbK_p4 AGGATTCGAACCTCCGAGTA AAAAAGCATAAGCCTCGGG 50.0 47.4 57.6 55.9 481-501 bp 1.0 54.7
trnQ-UUG-psbK_p5 AAGGATTCGAACCTCCGAGT AAAAAGCATAAGCCTCGGG 50.0 47.4 58.4 55.9 482-502 bp 1.0 54.6