Hotspot

trnK-UUU-rps16

LSC · 4041-4718 · 678 bp

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Pi 0.00426
MarkerSeek score 62.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
678
variable_sites
11
pi
0.00426
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 763-786 bp 1.0 80.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 762-785 bp 1.0 80.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 775-798 bp 1.0 79.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAATGGCGCTCAAC 50.0 50.0 58.2 58.3 775-798 bp 1.0 79.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 774-797 bp 1.0 79.7