Hotspot

ycf1

SSC · 121145-126352 · 5208 bp

Back to Alyssum

Pi 0.0518

MarkerSeek score 73.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5208
variable_sites: 417
pi: 0.0518
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTACGCCTTTGGGAACG	GGAAGAATTGGAAGCCGACT	50.0	50.0	58.0	57.9	240 bp	0.5	61.4
ycf1_p2	GTTTTACGCCTTTGGGAACG	TGGAAGAATTGGAAGCCGAC	50.0	50.0	58.0	58.2	241 bp	0.5	60.9
ycf1_p3	TCGGATAGTTGAACCCCAGT	GGAAGAATTGGAAGCCGACT	50.0	50.0	58.1	57.9	663 bp	0.5	60.7
ycf1_p4	GTTTTACGCCTTTGGGAACG	GTCCCAATGGAACCCGAAAT	50.0	50.0	58.0	58.2	209 bp	0.5	60.4
ycf1_p5	TCGGATAGTTGAACCCCAGT	GTCCCAATGGAACCCGAAAT	50.0	50.0	58.1	58.2	632 bp	0.5	60.1