Hotspot

rpl16

LSC · 78573-79975 · 1403 bp

Back to Alyssum

Pi 0.0383

MarkerSeek score 73.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1403
variable_sites: 82
pi: 0.0383
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2223-2313 bp	1.0	86.8
rpl16_p2	TAACGTCACCAATATGCGCA	TGAACAGGCGAATACAAGGG	45.0	50.0	58.0	57.9	1955-2045 bp	0.75	76.8
rpl16_p3	TAACGTCACCAATATGCGCA	GTATGGAGGGAATTGCACGT	45.0	50.0	58.0	58.0	2244-2334 bp	0.75	76.7
rpl16_p4	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2192-2282 bp	0.75	76.7
rpl16_p5	TAACGTCACCAATATGCGCA	TGCACGTGTTGAATGGATCA	45.0	45.0	58.0	58.1	1982 bp	0.25	49.0