Hotspot

ndhE-ndhG

SSC · 115446-115719 · 274 bp

Back to Alyssum

Pi 0.0432

MarkerSeek score 71.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 274
variable_sites: 19
pi: 0.0432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGACTCGTGATCAATCCA	TTTCGGTGGCTCGTCAATAA	45.0	45.0	56.7	57.5	333-381 bp	1.0	74.0
ndhE-ndhG_p2	TTCGACTCGTGATCAATCCA	ATTTCGGTGGCTCGTCAATA	45.0	45.0	56.7	57.3	334-382 bp	1.0	72.2
ndhE-ndhG_p3	TCGACTCGTGATCAATCCAT	TTTCGGTGGCTCGTCAATAA	45.0	45.0	56.5	57.5	332-380 bp	1.0	72.2
ndhE-ndhG_p4	TTTCGACTCGTGATCAATCCA	TTTCGGTGGCTCGTCAATAA	42.9	45.0	57.4	57.5	334-382 bp	1.0	71.8
ndhE-ndhG_p5	TCGACTCGTGATCAATCCAT	ATTTCGGTGGCTCGTCAATA	45.0	45.0	56.5	57.3	333-381 bp	1.0	70.4