Hotspot

trnT-ACA-trnL-UUA

LSC · 45073-45851 · 779 bp

Back to Alyssum

Pi 0.0527

MarkerSeek score 86.30

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 61
pi: 0.0527
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	55.0	50.0	57.9	57.8	806-911 bp	1.0	87.0
trnT-ACA-trnL-UUA_p2	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	50.0	50.0	57.8	57.8	838-943 bp	1.0	85.9
trnT-ACA-trnL-UUA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCAATTCCGCCATA	55.0	50.0	57.9	57.5	793-898 bp	1.0	84.6
trnT-ACA-trnL-UUA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	831-936 bp	1.0	84.4
trnT-ACA-trnL-UUA_p5	CTAACCTCTGAGCTAAGCGG	ATTAAGTCCGCAGCGTCTAC	55.0	50.0	57.5	57.8	798-903 bp	1.0	84.1