Hotspot

atpH-atpI

LSC · 12979-13451 · 473 bp

Back to Alyssum

Pi 0.0296

MarkerSeek score 67.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 22
pi: 0.0296
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	582-601 bp	1.0	84.8
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	622-641 bp	1.0	84.2
atpH-atpI_p3	AACCGAAGCAGCAGAAATCA	TTTTTGCCACTTTAGCTGCG	45.0	45.0	58.1	57.9	559-578 bp	1.0	84.2
atpH-atpI_p4	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	599-618 bp	1.0	83.6
atpH-atpI_p5	AGGTCCAATAGAAGCAAGCC	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.6	58.0	624-643 bp	1.0	81.8