Hotspot

trnG-GGA-trnR-AGA

LSC · 8893-9068 · 176 bp

Back to Alyssum

Pi 0.048

MarkerSeek score 67.00

Primer available yes

Label trnG-GGA-trnR-AGA

Feature Metadata

label_name: trnG-GGA-trnR-AGA
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 13
pi: 0.048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GGA-trnR-AGA_p1	AGCCTTCCAAGCTAACGATG	TGGTATTGCGTCCAATAGGA	50.0	45.0	58.0	56.3	274-303 bp	1.0	74.0
trnG-GGA-trnR-AGA_p2	ACCCTTAGCCTTCCAAGCTA	TGGTATTGCGTCCAATAGGA	50.0	45.0	58.0	56.3	280-309 bp	1.0	73.9
trnG-GGA-trnR-AGA_p3	CGACGTCGACTAAAACCCTT	TGGTATTGCGTCCAATAGGA	50.0	45.0	57.9	56.3	294-323 bp	1.0	73.5
trnG-GGA-trnR-AGA_p4	AAACCCTTAGCCTTCCAAGC	TGGTATTGCGTCCAATAGGA	50.0	45.0	58.1	56.3	282-311 bp	1.0	73.5
trnG-GGA-trnR-AGA_p5	AGCCTTCCAAGCTAACGATG	TTGGTATTGCGTCCAATAGGA	50.0	42.9	58.0	57.1	275-304 bp	1.0	72.0