Hotspot

rps16-trnQ-CAA

LSC · 5309-5988 · 680 bp

Back to Alyssum

Pi 0.0471
MarkerSeek score 78.70
Primer available yes
Label rps16-trnQ-CAA

Feature Metadata

label_name
rps16-trnQ-CAA
feature_type
igs
region
LSC
length_bp
680
variable_sites
50
pi
0.0471
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-CAA_p1 GCAAGCTCTATACGCGTCTT CAAAACCCTAGCACCACTGT 50.0 50.0 58.2 58.0 1116 bp 0.25 48.6
rps16-trnQ-CAA_p2 GCAAGCTCTATACGCGTCTT TGGCGACTAAAAAGGTGCTT 50.0 45.0 58.2 58.0 1469 bp 0.25 48.5
rps16-trnQ-CAA_p3 GCAAGCTCTATACGCGTCTT TGTCAAAACCCTAGCACCAC 50.0 50.0 58.2 58.0 1119 bp 0.25 48.5
rps16-trnQ-CAA_p4 GCAAGCTCTATACGCGTCTT TTGGCGACTAAAAAGGTGCT 50.0 45.0 58.2 58.0 1470 bp 0.25 48.5
rps16-trnQ-CAA_p5 GCGCCCTTTTCAGTAAATCG CAAAACCCTAGCACCACTGT 50.0 50.0 57.8 58.0 986 bp 0.25 48.5