Hotspot

trnH-CAC-psbA

LSC · 76-452 · 377 bp

Back to Alyssum

Pi 0.118

MarkerSeek score 75.90

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 70
pi: 0.118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	402-435 bp	1.0	90.5
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	417-450 bp	1.0	90.4
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	420-453 bp	1.0	90.3
trnH-CAC-psbA_p4	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	403-436 bp	1.0	90.3
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	418-451 bp	1.0	90.2