Hotspot

ycf2

IRa · 143791-150663 · 6873 bp

Back to Alternanthera

Pi 0.00845

MarkerSeek score 51.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6873
variable_sites: 58
pi: 0.00845
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGCAAGAACCCCGAATCATT	GCATCTTGTTCTATCCTTGATCA	45.0	39.1	57.8	56.4	263 bp	1.0	44.3
ycf2_p2	CTGCAAGAACCCCGAATCAT	GCATCTTGTTCTATCCTTGATCA	50.0	39.1	58.2	56.4	264 bp	1.0	44.0
ycf2_p3	GAAATGACCCGGCCCAATAG	GCATCTTGTTCTATCCTTGATCA	55.0	39.1	58.7	56.4	214 bp	1.0	43.4
ycf2_p4	ATAAATGATCCGCTTGCCCC	GCATCTTGTTCTATCCTTGATCA	50.0	39.1	58.4	56.4	234 bp	1.0	43.3
ycf2_p5	CATAAATGATCCGCTTGCCC	GCATCTTGTTCTATCCTTGATCA	50.0	39.1	57.3	56.4	235 bp	1.0	43.1