Hotspot

ndhF

SSC · 109801-112038 · 2238 bp

Back to Alternanthera

Pi 0.0179

MarkerSeek score 53.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 40
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAATCGTCCGACGCGATATA	TGACCGATTCGAGCTTGATG	50.0	50.0	57.5	58.1	2855-2896 bp	1.0	77.9
ndhF_p2	GAATCGTCCGACGCGATATA	GACCGATTCGAGCTTGATGA	50.0	50.0	57.5	57.8	2854-2895 bp	1.0	76.8
ndhF_p3	GAATCGTCCGACGCGATATA	TTTGACCGATTCGAGCTTGA	50.0	45.0	57.5	57.6	2857-2898 bp	1.0	74.9
ndhF_p4	GAATCGTCCGACGCGATATAT	TGACCGATTCGAGCTTGATG	47.6	50.0	58.0	58.1	2855-2896 bp	1.0	73.7
ndhF_p5	GAATCGTCCGACGCGATATA	TTGACCGATTCGAGCTTGAT	50.0	45.0	57.5	57.3	2856-2897 bp	1.0	73.1