Hotspot

rpl32-trnL

SSC · 113004-113675 · 672 bp

Back to Alternanthera

Pi 0.0402

MarkerSeek score 68.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 672
variable_sites: 27
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	CATCAAGCTCGAATCGGTCA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.1	58.1	1434-1453 bp	1.0	82.8
rpl32-trnL_p2	CATCAAGCTCGAATCGGTCA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.1	58.1	1436-1455 bp	1.0	82.8
rpl32-trnL_p3	CATCAAGCTCGAATCGGTCA	TTCAGTTAAGAAGCCCGACG	50.0	50.0	58.1	57.9	1905-1924 bp	1.0	82.2
rpl32-trnL_p4	GTATTCGGCGGCGTTAAAAG	CTCGCACTGCTTCCTAAGAG	50.0	55.0	57.9	58.1	810-811 bp	1.0	81.7
rpl32-trnL_p5	GTATTCGGCGGCGTTAAAAG	TTCAGTTAAGAAGCCCGACG	50.0	50.0	57.9	57.9	1281-1282 bp	1.0	81.2