Hotspot

ndhF-rpl32

SSC · 112039-112844 · 806 bp

Back to Alternanthera

Pi 0.039

MarkerSeek score 73.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 806
variable_sites: 30
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGTAAGCGGATCGAGCAAG	CTTTTAACGCCGCCGAATAC	50.0	50.0	58.1	57.9	1142-1177 bp	1.0	83.6
ndhF-rpl32_p2	GAAGTAAGCGGATCGAGCAA	CTTTTAACGCCGCCGAATAC	50.0	50.0	58.1	57.9	1143-1178 bp	1.0	83.6
ndhF-rpl32_p3	AAGCGGATCGAGCAAGTATC	CTTTTAACGCCGCCGAATAC	50.0	50.0	57.9	57.9	1138-1173 bp	1.0	83.1
ndhF-rpl32_p4	GAAGGGGAAATTGAGCCGAT	CTTTTAACGCCGCCGAATAC	50.0	50.0	57.9	57.9	1601-1636 bp	1.0	82.6
ndhF-rpl32_p5	ACGGTTAGTCACAAACGCTT	CTTTTAACGCCGCCGAATAC	45.0	50.0	58.1	57.9	1446 bp	0.5	58.9