Hotspot

rps3-rps19

LSC · 83780-84463 · 684 bp

Back to Alternanthera

Pi 0.0322

MarkerSeek score 67.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 22
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CCAAGTCTGAAACCAAGTGGA	AATTTCCGGGGACATGCAAA	47.6	45.0	58.1	58.4	767 bp	1.0	70.9
rps3-rps19_p2	CCAAGTCTGAAACCAAGTGGA	ATTTCCGGGGACATGCAAAA	47.6	45.0	58.1	58.4	766 bp	1.0	70.9
rps3-rps19_p3	CCAAGTCTGAAACCAAGTGGA	AAATTTCCGGGGACATGCAA	47.6	45.0	58.1	58.4	768 bp	1.0	70.8
rps3-rps19_p4	TGGTTGTGAAAACCAAAGGGA	AATTTCCGGGGACATGCAAA	42.9	45.0	58.2	58.4	807 bp	1.0	69.9
rps3-rps19_p5	TGGTTGTGAAAACCAAAGGGA	ATTTCCGGGGACATGCAAAA	42.9	45.0	58.2	58.4	806 bp	1.0	69.9