Hotspot

rpl16-rps3

LSC · 82973-83122 · 150 bp

Back to Alternanthera

Pi 0.0467

MarkerSeek score 68.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 7
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCGACGAGTCACACA	CTCTACAAACCATTCGGGCA	45.0	50.0	58.0	57.9	282 bp	1.0	81.0
rpl16-rps3_p2	ACAAAAACCGACGAGTCACA	CTCTACAAACCATTCGGGCA	45.0	50.0	58.0	57.9	284 bp	1.0	81.0
rpl16-rps3_p3	CCGACGAGTCACACACTAAG	CTCTACAAACCATTCGGGCA	55.0	50.0	58.0	57.9	277 bp	1.0	80.9
rpl16-rps3_p4	CAAAAACCGACGAGTCACAC	CTCTACAAACCATTCGGGCA	50.0	50.0	57.6	57.9	283 bp	1.0	78.2
rpl16-rps3_p5	GACGAGTCACACACTAAGCA	CTCTACAAACCATTCGGGCA	50.0	50.0	57.6	57.9	275 bp	1.0	77.8