Hotspot

psaJ-rpl33

LSC · 67274-67712 · 439 bp

Back to Alternanthera

Pi 0.0349

MarkerSeek score 75.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 15
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	CTGGAAACGCCCCTTGATAA	50.0	50.0	58.0	57.9	648-649 bp	1.0	81.7
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	GAAACGCCCCTTGATAACCT	50.0	50.0	58.0	57.9	645-646 bp	1.0	81.7
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TGGAAACGCCCCTTGATAAC	50.0	50.0	58.0	58.2	647-648 bp	1.0	81.3
psaJ-rpl33_p4	CAGATGCGTTGACATTCCCT	CTGGAAACGCCCCTTGATAA	50.0	50.0	58.3	57.9	563-564 bp	1.0	80.4
psaJ-rpl33_p5	CAGATGCGTTGACATTCCCT	GAAACGCCCCTTGATAACCT	50.0	50.0	58.3	57.9	560-561 bp	1.0	80.4