Hotspot

rpoC2-rpoC1

LSC · 20643-20828 · 186 bp

Back to Alternanthera

Pi 0.0552
MarkerSeek score 71.50
Primer available yes
Label rpoC2-rpoC1

Feature Metadata

label_name
rpoC2-rpoC1
feature_type
igs
region
LSC
length_bp
186
variable_sites
10
pi
0.0552
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rpoC1_p1 GGCAGTTCCATCTATCGCTT AGGATTTTGTCAAGCCTGCT 50.0 45.0 57.8 57.7 260-265 bp 1.0 80.2
rpoC2-rpoC1_p2 GTGAAAGACCAAATTGGCCC AGGATTTTGTCAAGCCTGCT 50.0 45.0 57.5 57.7 236-241 bp 1.0 79.0
rpoC2-rpoC1_p3 AGTCGTTTCATGGCAGTTCC AGGATTTTGTCAAGCCTGCT 50.0 45.0 58.5 57.7 271-276 bp 1.0 78.2
rpoC2-rpoC1_p4 AAAGACCAAATTGGCCCGTT AGGATTTTGTCAAGCCTGCT 45.0 45.0 58.6 57.7 233-238 bp 1.0 78.1
rpoC2-rpoC1_p5 GGCAGTTCCATCTATCGCTT GGATTTTGTCAAGCCTGCTC 50.0 50.0 57.8 57.4 259-264 bp 1.0 77.5