Hotspot

psbI-trnS

LSC · 8131-8329 · 199 bp

Back to Alternanthera

Pi 0.0556

MarkerSeek score 78.30

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 10
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	GTAATCCTGGACGCGAAGAA	GTCATTCGTACCGAGGGTTC	50.0	55.0	57.7	58.1	240-259 bp	1.0	84.2
psbI-trnS_p2	GTAATCCTGGACGCGAAGAA	AGTCATTCGTACCGAGGGTT	50.0	50.0	57.7	58.4	241-260 bp	1.0	81.3
psbI-trnS_p3	GTAATCCTGGACGCGAAGAA	TTTTCAACGGGGAGAGATGG	50.0	50.0	57.7	57.5	301-320 bp	1.0	80.3
psbI-trnS_p4	GTAATCCTGGACGCGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.7	58.6	286-305 bp	1.0	79.3
psbI-trnS_p5	GTAATCCTGGACGCGAAGAA	GAGTCATTCGTACCGAGGGT	50.0	55.0	57.7	58.9	242-261 bp	1.0	77.7