Hotspot

rps15-trnN-GUU

IRa · 139483-146544 · 7062 bp

Back to Alstonia

Pi 0.136
MarkerSeek score 74.00
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
IRa
length_bp
7062
variable_sites
1162
pi
0.136
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.84
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CGACGCAGATTCACCCTATT CCAATCCACTCGATTTCAACC 50.0 47.6 57.8 57.2 533 bp 0.333333 37.4
rps15-trnN-GUU_p2 CGACGCAGATTCACCCTATT TGCCAGACTCAATACACAAGT 50.0 42.9 57.8 57.2 642 bp 0.333333 37.4
rps15-trnN-GUU_p3 TACGACGCAGATTCACCCTA CCAATCCACTCGATTTCAACC 50.0 47.6 58.2 57.2 535 bp 0.333333 37.2
rps15-trnN-GUU_p4 TACGACGCAGATTCACCCTA TGCCAGACTCAATACACAAGT 50.0 42.9 58.2 57.2 644 bp 0.333333 37.2
rps15-trnN-GUU_p5 TGCAGGACTCATTGATGCAG CCAATCCACTCGATTTCAACC 50.0 47.6 58.3 57.2 916 bp 0.333333 37.2