Hotspot

ndhF-rpl32

SSC · 128733-129380 · 648 bp

Back to Alstonia

Pi 0.12

MarkerSeek score 84.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 648
variable_sites: 111
pi: 0.12
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGAGCCACCCGTATGGATAT	TTGAACGCTGTCCAATACCC	50.0	50.0	58.0	58.2	768-776 bp	0.666667	77.0
ndhF-rpl32_p2	CAAGAGGTCGTGTGAACCAA	TTGAACGCTGTCCAATACCC	50.0	50.0	58.1	58.2	1248-1256 bp	0.666667	74.6
ndhF-rpl32_p3	ATGAGCCACCCGTATGGATA	TTGAACGCTGTCCAATACCC	50.0	50.0	58.0	58.2	769 bp	0.333333	51.7
ndhF-rpl32_p4	TATGAGCCACCCGTATGGAT	TTGAACGCTGTCCAATACCC	50.0	50.0	58.0	58.2	770 bp	0.333333	51.7
ndhF-rpl32_p5	GAATTTTCTGTTGCCGTCGG	TTGAACGCTGTCCAATACCC	50.0	50.0	58.0	58.2	844 bp	0.333333	51.6