Hotspot

trnN-GUU-rps15

IRb · 116054-123115 · 7062 bp

Back to Alstonia

Pi 0.0675

MarkerSeek score 56.50

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 7062
variable_sites: 121
pi: 0.0675
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.17
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AAGAGAAGAAAGGGCGCAAA	TTTGCAGACCTTCCCTTTCC	45.0	50.0	58.0	58.0	1217 bp	0.333333	53.1
trnN-GUU-rps15_p2	AAGAGAAGAAAGGGCGCAAA	GACCATCGCGGGACTATTTT	45.0	50.0	58.0	58.1	1278 bp	0.333333	52.8
trnN-GUU-rps15_p3	AAGAGAAGAAAGGGCGCAAA	TCCCTTTCCATTGAGTCCCT	45.0	50.0	58.0	57.9	1206 bp	0.333333	52.7
trnN-GUU-rps15_p4	AAGAGAAGAAAGGGCGCAAA	TATGACCATCGCGGGACTAT	45.0	50.0	58.0	58.1	1281 bp	0.333333	52.5
trnN-GUU-rps15_p5	AAGAGAAGAAAGGGCGCAAA	CGAGAGGTCCCCCTTAAGAA	45.0	55.0	58.0	58.1	1005 bp	0.333333	52.1