Hotspot

ycf15-trnL-CAA

IRb · 93882-101687 · 7806 bp

Back to Alstonia

Pi 0.0628

MarkerSeek score 53.80

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 7806
variable_sites: 37
pi: 0.0628
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	ATACCCGTCACATGCTTTCC	TGAGGAGAGCTTTGTGGAGA	50.0	50.0	58.0	58.0	1309 bp	0.333333	52.9
ycf15-trnL-CAA_p2	ATACCCGTCACATGCTTTCC	AGAAGGGGTTGGAGTCGTTA	50.0	50.0	58.0	58.0	1191 bp	0.333333	52.9
ycf15-trnL-CAA_p3	TCGTTCCAGTGAAAAGGCAA	TGAGGAGAGCTTTGTGGAGA	45.0	50.0	58.0	58.0	1917 bp	0.333333	52.8
ycf15-trnL-CAA_p4	TTCGTTCCAGTGAAAAGGCA	TGAGGAGAGCTTTGTGGAGA	45.0	50.0	58.0	58.0	1918 bp	0.333333	52.8
ycf15-trnL-CAA_p5	ATACCCGTCACATGCTTTCC	CATTCCGATGTAGTGGTGCA	50.0	50.0	58.0	58.0	1210 bp	0.333333	52.8